Recombinant Mouse Anti-BNP Antibody (106.3)
CAT#: PABX-017
Anti-BNP antibody is a Mouse antibody of IgG1 class that binds to an BNP. This antibody binds an epitope spanning residues 5-13 of the mature bioactive peptide.
Tested Data
Published Data
Gene Expression
ELISA
Figure 1 Anti-BNP Antibody (PABX-017) in ELISA
ELISA: Recombinant Mouse Anti-BNP Antibody (106.3) [PABX-017] detects BNP peptide.
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FuncS
Figure 1 Equilibrium binding titration of Alexa488-BNP 5-13 with mAb 106.3 at various temperatures.
The dissociation constants are: 0.05 nM, 0.07 nM, 0.2 nM, 0.6 nM, 1.2 nM, 3.4 nM, 7.2 nM.
Longenecker, K. L., Ruan, Q., Fry, E. H., Saldana, S. C., Brophy, S. E., Richardson, P. L., & Tetin, S. Y. (2009). Crystal structure and thermodynamic analysis of diagnostic mAb 106.3 complexed with BNP 5‐13 (C10A). Proteins: Structure, Function, and Bioinformatics, 76(3), 536-547.
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Subcellular Location
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/77324/1928_E11_1_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (BEWO, CACO-2, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120937-NPPB
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native Human BNP
- Species Reactivity
- Human
- Clone
- 106. 3
- Applications
- FuncS
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- This antibody has been reported for use in Functional Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.
Target
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Great for ELISA Assay
Using the recombinant mouse anti-BNP antibody (106.3), we have detected the high concentration BNP peptide. This excited result provided valuable guidance and support for our work in the future.
Suitable for IF Staining
Benefited by this antibody, our immunofluorescence staining experiment obtained ideal results. Target protein showed accurate localization to vesicles in the cell line that used in our experiment. It displayed strong fluorescence signal in a relatively short exposure time that can be accurately identified under the confocal microscope.
Q&As
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What is the maximum size of the recombinant mouse anti-BNP antibody (106.3)?
A: The maximum size of the recombinant mouse anti-BNP antibody (106.3) is 1g, and the purity is more than 95% as determined by analysis by SDS-PAGE.
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Would I use the recombinant mouse anti-BNP antibody (106.3) for human primary cells?
A: Yes, the recombinant mouse anti-BNP antibody (106.3) can be used for human primary cells, and we have proven this.
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Can I use the recombinant mouse anti-BNP antibody (106.3) in human cell line for IF?
A: Yes, the recombinant mouse anti-BNP antibody (106.3) can be used in human cell line for IF. The target protein is located at cytosol and vesicles.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABX-017, RRID: AB_3111692)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| PABX-017-S (P) | Recombinant Mouse Anti-BNP Antibody scFv Fragment (106.3) | WB, ELISA, IF, FuncS | scFv |
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